dPEG® Building Blocks:
Quanta BioDesign's popular line of dPEG® building blocks provides SuperHydrophilic™ linkers of various molecular sizes and designs. The reagents contain reactive groups or protected functional groups for almost any application. They can be used to modify particles, create linker arms during peptide or oligonucleotide synthesis, or construct drug payload designs for antibody-drug conjugates (ADCs).
Our peptide modification products offers Cbz-, Fmoc-, and boc-protected amino-dPEG®x-acid compounds. Some of our Fmoc-protected products are available as preactivated (NHS or TFP esters) products. We also provide methoxytrityl-protected thiol compounds that permit conversion of an amine to a thiol and Fmoc-protected aminooxy products for customers who need to form oxime bonds. Finally, we offer two novel products that permit easy insertion of a biotin label into a peptide chain. Discover "the dPEG® difference" with Quanta BioDesign's line of unique building blocks.
• Acid-dPEG®x-NHS ester
• Amino-dPEG®x-t-butyl ester
• Bis-dPEG®x, half benzyl half NHS ester
• Fmoc-N-amido-dPEG®x-NHS ester
• Hydroxy-dPEG®x-t-butyl ester
• Hydroxy-dPEG®x-TFP ester
• Fmoc-N-amido-dPEG®x-TFP ester
• Phthalimidooxy-dPEG®x-NHS ester-acid
dPEG® Delivery Reagents:
Quanta BioDesign's line of payload delivery reagents includes three important product categories: DOTA-dPEG® bifunctional chelators, Phospholipid dPEG® derivatives; and our exclusive Sidewinder™ dPEG® constructs. Our DOTA products are useful for targeted radioimaging and radiotherapy applications with labeled antibodies. We designed the phospholipid category of products for use as liposomal and micellar drug delivery vehicles. In addition, our unique Sidewinder™ product line permits highly controlled construction of drug delivery systems with high drug-to-antibody ratios (DARs). With all of these delivery agents, "the dPEG® difference" provides enhanced hydrophilicity and biocompatibility so that modified antibodies retain stability and display low nonspecificity.
dPEG® Conjugation Reagents:
The process of bioconjugation often uses reactive crosslinkers to couple two molecules together through a crossbridge or linker arm. The use of traditional aliphatic crosslinkers can add unwanted hydrophobicity to modified biomolecules and cause aggregation and nonspecific interactions. By contrast, crosslinking reagents made with our novel, hydrophilic dPEG® groups produce water-soluble compounds, which create conjugates having greater biocompatibility and extremely low nonspecific binding properties.
Our selection of dPEG® crosslinkers includes homobifunctional, heterobifunctional, and branched chain architectures with a wide variety of reactive groups. All of the crosslinkers contain our single molecular weight dPEG® chains as crossbridges. The choice of dPEG® spacers can span molecular lengths from 2 to 48 ethylene glycol repeating units to satisfy virtually any application.
• Heterobifunctional Reagents: Amine and Thiol Reactive dPEG® Reagents
• Heterobifunctional Reagents: dPEG® Reagents for Hydrazone Bond Formation
• Heterobifunctional: Monoprotected dPEG® Reagents
• Homobifunctional Reagents: Amine Reactive dPEG® Reagents
• Homobifunctional: Thiol Reactive dPEG® Reagents
• Homotetrafunctional: Thiol Reactive dPEG® Reagents
• Other Thiol Reactive
dPEG® Click Reagents
Whether you need dPEG® compounds for strain-promoted (SPAAC), copper-catalyzed (CuAAC), or ruthenium-catalyzed (RuAAC) reactions, your click chemistry is enabled as never before by our water-soluble, non-immunogenic dPEG® products. Even hydrophobic cyclooctyne compounds are made more hydrophilic by incorporating SuperHydrophilic™ dPEG® linkers. Click now to empower your chemistry!
• Click dPEG® Azide Crosslinkers
• Click dPEG® Biotinylation Reagents
• Click dPEG® Terminal Alkynes
• Click dPEGylation® Reagents
• Click Fluorescent Dyes
• Copper-Free Click dPEG® Crosslinkers
• Crosslinkers: dPEG® Alkynes
• DBCO-dPEG® Reagents - Hydrophilic Copper-Free Click Reagents
The dPEG®-Biotin Advantage
Biotinylation is one of the most widely used bioconjugation techniques. It can add a biotin tag to antibodies, proteins, and other molecules for high-affinity binding with streptavidin reagents. Our dPEG® spacers create a significant advantage over aliphatic biotin compounds, such as the popular NHS-LC-biotin reagent. The SuperHydrophilic™ dPEG® spacers prevent antibody or protein denaturation, aggregation, or precipitation, which is often a problem when using hydrophobic biotinylation compounds. Discover the dPEG® difference with our water-soluble biotinylation reagents and our wide selection of conjugation chemistries.
• Amine Reactive dPEG® Biotinylation Reagents
• Biotin-dPEG®x-azide for Click Chemistry
• Biotinidase Resistant Biotin
• Carbonyl Reactive dPEG® Biotinylation Reagents
• Other dPEG® Biotinylation Reagents
• Photoaffinity dPEG® Biotinylation Reagent
• Thiol Reactive dPEG® Biotinylation Reagents
Discover the dPEG® difference with our broad range of architectures and functional and reactive groups for the chemical modification of small molecules, biomolecules, and nanoparticle surfaces. Improve biocompatibility and increase the water solubility of hydrophobic molecules by adding a discrete dPEG® modifier. Use hydrophilic dPEG® thiolation reagents to create thiols for conjugation. Modify with branched-chain dPEG® reagents to change pharmacokinetics. Coat particle or planar surfaces with dPEG® modification reagents to create functionality and mask underlying hydrophobicity.
Bioconjugate Chemistry Publication:
Linker Architectures as Steric Auxiliaries for Altering Enzyme-Mediated Payload Release from Bioconjugates
• Amine Reactive dPEG® Reagents
• Branched dPEG® PK Modifiers
• dPEG® Thiolation Reagents: Converting an Amine to a Protected Thiol
• Metal Surface dPEG® Modification Reagents
• Other Reactivities for dPEG® Modifications
• Reagents to Extend Carboxyl with dPEG® Spacers
• SuperHydrophilicTM dPEG® Chemical Modification Reagents
• Thiol Reactive dPEG® Modification Reagents
By Greg T Hermanson
August 2013 / Print Book ISBN: 9780123822390 / eBook ISBN: 9780123822406
- The definitive source for proven methods and protocols for preparing bioconjugates
- Provides step-by-step protocols
- Features full color illustrations, molecular models, and reactions
- This edition includes an extensive introduction into the vast field of bioconjugation and one of the most thorough overviews of immobilization chemistry ever presented
Bioconjugate Techniques is the essential guide to the modification and crosslinking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions, with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, synthetic polymers, and particles and surfaces of many types.